Taking the bus is the cheapest way to travel from Florence to Venice. Bus companies that travel from Florence to Venice include Flixbus and Baltour. Traveling from Florence to Venice by bus takes approximately 3:45 h. One bus departs in the morning at 5:25 a.m., while the other departs in the evening at 5:25 p.m. Buses usually depart from Florence's Piazzale Montelungo and arrive at Venice's Tronchetto people mover.

Taking the train is the fastest way to travel from Florence to Venice. Both Trenitalia and Italo both operate direct trains between Florence and Venice. Traveling from Florence to Venice by train takes as little as 1:50 h. Trains usually run from Florence to Venice every hour between 8:30 a.m. and 9:45 p.m., departing from either Florence's S.M. Novella or Campo di Marte train stations and arriving at Venice's S. Lucia train station. Overnight train journeys are also available.

Taking the train is the most popular way to travel from Florence to Venice. Taking the train is almost 2 hours faster than traveling from Florence to Venice by bus, while only being €10 more expensive on average. Both taking the train and bus are significantly more efficient in terms of both travel time and money when compared to flying between these two Italian cities.