Taking the bus or a rideshare is the cheapest way to travel from Florence to Pisa. Terravision buses travel from Florence to Pisa once an hour every day. The earliest bus traveling from Florence to Pisa usually depart from Florence's Piazza Montelungo at 3:40 a.m. while the final bus departs at 9:00 p.m. Traveling from Florence to Pisa by bus takes approximately 1 hour to reach Pisa Airport, so travelers will need to take a further bus to reach Pisa's city center. Fortunately, this bus (the PisaMover) only takes an additional 10 minutes. Ridesharing services like BlaBlaCar that travel from Florence to Pisa also take approximately 1:10 h to arrive in Pisa.

Taking the train is the fastest way to travel from Florence to Pisa. Trains traveling from Florence to Pisa depart from Florence's Santa Maria Novella station and arrive at Pisa Centrale train station. All trains traveling from Florence to Pisa are operated by Trenitalia. Some of Trenitalia's Frecciabianca trains cover the short distance in just 48 minutes, while slower regional Trenitalia services can take almost 3 hours to arrive in Pisa. Over 50 trains travel from Florence to Pisa every day, with the first train departing Florence at 4:30 a.m.

Taking the train is the most popular way to travel from Florence to Pisa. Taking a train is the fastest way to travel from Florence to Pisa and isn't significantly more expensive than taking the bus. Over 50 Trenitalia trains traveling from Florence to Pisa every day. Up to 4 trains depart per hour during peak hours.