- Must visit
Florida Museum of Natural History
Natural history museum with Florida fossils, butterfly exhibits, and family-friendly science displays on the University of Florida campus.
Cheapest price on Oct 09
$153
Average ticket price on Oct 09
$153
Fastest journey on Oct 09
22 h 40 m
Average journey duration on Oct 09
22 h 40 m
Buses on Oct 09
1
Distance
340 km
The best way to find a cheap bus ticket from Fort Myers, FL to Gainesville, FL is to book your journey as far in advance as possible and to avoid traveling at rush hour.
The average ticket from Fort Myers, FL to Gainesville, FL will cost around $153 if you buy it on the day, but the cheapest tickets can be found for only $153.
It's difficult to get from Fort Myers, FL to Gainesville, FL without transferring at least once.


Buses from Fort Myers, FL to Gainesville, FL run on average 5 times per day, taking around 7h 53m. Cheap bus tickets for this journey start at $46 (€39) if you book in advance.
There are 1 buses per day. The earliest bus runs at 00:25, the last at 22:40. The fastest bus covers the 211 miles (340 km) distance in 5h 5m.
Distance 211 miles (340 km) |
Average bus duration 7h 53m |
Cheapest ticket price $46 (€39) |
Buses per day 5 |
Direct buses 1 |
Fastest bus 5h 5m |
First bus 12:25 AM |
Last bus 10:40 PM |
Cheapest Month Aug |
Cheapest Day Wed |
High Season Jul - Sep |
Low Season Apr - Jun |
Busiest Day Tue |
Least Busy Day Tue |
| Operator | Departure Time | Arrival Time | Duration | Departure days | Cheapest price |
|---|---|---|---|---|---|
Operator Pinkbus | Departure Time 11:55AM | Arrival Time 05:00PM | Duration 5h05 | Departure days Thu | Cheapest price $99 |
Operator FlixBus | Departure Time 12:20PM | Arrival Time 11:45PM | Duration 11h25 | Departure days Thu | Cheapest price $79 |
Operator Pinkbus | Departure Time 03:55PM | Arrival Time 09:00PM | Duration 5h05 | Departure days Tue | Cheapest price $102 |
Operator Pinkbus | Departure Time 04:55PM | Arrival Time 10:00PM | Duration 5h05 | Departure days Wed | Cheapest price $90 |
Operator Flixbus USA | Departure Time 06:20PM | Arrival Time 05:00PM | Duration 22h40 | Departure days Fri, Sat, Sun | Cheapest price $153 |
Operator Greyhound | Departure Time 06:30PM | Arrival Time 11:25AM | Duration 16h55 | Departure days Thu | Cheapest price $103 |
Operator FlixBus | Departure Time 06:55PM | Arrival Time 11:59AM | Duration 17h04 | Departure days Thu | Cheapest price $79 |
The table below shows live departures for Fort Myers, FL to Gainesville, FL buses for today, Thursday, October 8. You can also manually refresh the results below to see updated bus journeys.
| Provider | Departs | Duration | Arrives | Transfers | Tickets |
|---|---|---|---|---|---|
![]() | 11:55 AM Fort Myers, Old Luckett Drive, FL | 5h05 | 5:00 PM Gainesville, Gale Lemerand Dr (Commuter Lot Parking Garage), FL | 0 transfersDirect | |
FlixBus · FlixBus | 12:20 PM Fort Myers, Rosa Parks Transit Center | 11h25 | 11:45 PM Gainesville, Rosa Parks Transfer station | 1 transfer | |
FlixBus+ + FlixBus | 6:30 PM Fort Myers, Rosa Parks Transit Center | 16h55 | 11:25 AM Gainesville, Rosa Parks Transfer station | 2 transfers | |
FlixBus · FlixBus · FlixBus | 6:55 PM Fort Myers, Rosa Parks Transit Center | 17h04 | 11:59 AM Gainesville, Rosa Parks Transfer station | 2 transfers | |
FlixBus+![]() | 6:55 PM Fort Myers, Rosa Parks Transit Center | 12h55 | 7:50 AM Gainesville, NW 23rd St (North Walmart Supercenter), FL | 1 transfer |
Find all the dates and times for this journey
Discover the best of Gainesville, FL — top attractions, local food, transport tips, budget advice, and currency essentials. Plan your perfect Gainesville, FL trip today.
Natural history museum with Florida fossils, butterfly exhibits, and family-friendly science displays on the University of Florida campus.
University art museum featuring global collections, rotating exhibitions, and strong photography and modern art presentations.
Tree-lined downtown district with historic buildings, murals, music venues, and an easy walk to shops, theaters, and public art.
Tree-lined downtown district with historic buildings, murals, music venues, and an easy walk to shops, theaters, and public art.
Large wetlands park with boardwalks, wildlife viewing, and broad views over marshes that attract birds, alligators, and photographers.
Lively student-centered district near UF with shops, nightlife, murals, and walkable streets that capture the city's college-town energy.
North Florida barbecue often features slow-smoked pork or brisket with tangy sauces, reflecting Southern pit traditions common at local gatherings and roadside smokehouses.
Fresh Gulf shrimp are a Florida staple, commonly boiled, fried, or served in baskets. Gainesville restaurants highlight this regional seafood from nearby Gulf waters.
This Florida classic layers tart Key lime juice, sweetened condensed milk, and a crumb crust. It is a signature state dessert found widely in Gainesville.
Long-running barbecue spot serving smoked meats, classic sides, and a casual local atmosphere near downtown.
Stylish downtown venue focused on modern Southern food, oysters, and cocktails in an energetic setting.
Refined Southern restaurant known for seasonal plates, cocktails, and a polished but relaxed downtown setting.
Moderate for U.S. college towns: hotels can rise on game days, dining is reasonable, and most visitors need a car or rideshares.
Tip 18-20% at restaurants, 15-20% for taxis and rideshares, and $1-2 per drink or coffee is appreciated.
Find answers to the most common questions about traveling from Fort Myers, FL to Gainesville, FL by bus. From journey times and ticket prices to direct connections, first and last departures, and even whether the route is scenic, our FAQs cover everything you need to plan your trip. Whether you’re looking for the fastest bus, the cheapest option, or tips for making the most of your visit at Gainesville, FL, this guide helps you travel smarter and with confidence.

Most Fort Myers, FL to Gainesville, FL buses use the Fort Myers, Rosa Parks Transit Center to Gainesville, Rosa Parks Transfer station station pair, with some services arriving at Gainesville, NW 23rd St (North Walmart Supercenter). Use this guide to compare Fort Myers, FL and Gainesville, FL bus stations on this route, check city-centre transport, alongside checking practicalities like bus station facilities and services.
Fort Myers, Rosa Parks Transit Center is 3 km from Fort Myers city centre. Public transport options include:
Fort Myers, Rosa Parks Transit Center has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Wi-Fi is available at Fort Myers, Rosa Parks Transit Center. ATMs are available at Fort Myers, Rosa Parks Transit Center.
Ticket office support is available at Fort Myers, Rosa Parks Transit Center.
Available at Fort Myers, Rosa Parks Transit Center: Parking, Taxis, WC.
Accessibility facilities are available at Fort Myers, Rosa Parks Transit Center: Wheelchair accessible routes provided.
Gainesville, Rosa Parks Transfer station has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Free Wi-Fi is available at the station. ATMs are available at Gainesville, Rosa Parks Transfer station.
Ticket office support is available at Gainesville, Rosa Parks Transfer station.
Available at Gainesville, Rosa Parks Transfer station: Parking, Taxis, WC.
Accessibility facilities are available at Gainesville, Rosa Parks Transfer station: The station is wheelchair accessible.
Gainesville, NW 23rd St (North Walmart Supercenter) is 7 km from Gainesville city centre. Public transport options include:
Gainesville, NW 23rd St (North Walmart Supercenter) has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Wi-Fi is available at Gainesville, NW 23rd St (North Walmart Supercenter). ATMs are available at Gainesville, NW 23rd St (North Walmart Supercenter).
Ticket office support is available at Gainesville, NW 23rd St (North Walmart Supercenter).
Available at Gainesville, NW 23rd St (North Walmart Supercenter): Parking, Taxis, WC.
Accessibility facilities are available at Gainesville, NW 23rd St (North Walmart Supercenter): Bus stop has basic accessibility features.
Gainesville has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Wi-Fi is available at Gainesville. ATMs are available at Gainesville.
Ticket office support is available at Gainesville.
Available at Gainesville: Parking, Taxis, WC.
Accessibility facilities are available at Gainesville: Accessible for persons with reduced mobility.
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