- Must visit
Florida Museum of Natural History
Natural history museum with Florida fossils, butterfly exhibits, and family-friendly science displays on the University of Florida campus.
Cheapest price on Sep 20
€40
Average ticket price on Sep 20
€40
Fastest journey on Sep 20
4 h 50 m
Average journey duration on Sep 20
4 h 50 m
Buses on Sep 20
1
Distance
135 km
The best way to find a cheap bus ticket from Daytona Beach, FL to Gainesville, FL is to book your journey as far in advance as possible and to avoid traveling at rush hour.
The average ticket from Daytona Beach, FL to Gainesville, FL will cost around €40 if you buy it on the day, but the cheapest tickets can be found for only €40.
On Sun, Sep 20, we found 1 buses from Daytona Beach, FL to Gainesville, FL, including 1 direct services.
On Sun, Sep 20 the direct buses cover the 135 km distance in an average of 4 h 50 m but if you time it right, some buses will get you there in just 4 h 50 m .
On Sun, Sep 20 the slowest buses will take 4 h 50 m and usually involve a change or two along the way, but you might be able to save a few pennies if you’re on a budget.


Omio recommends booking the bus as it is the most popular option from Daytona Beach, FL to Gainesville, FL among Omio's users. If you are prioritising price, take the bus, with an average ticket price of €43 For those with little time, consider booking a bus, which has an average total journey time of 3h 51m. Average ticket prices depend on the travel mode: a bus costs an average of €43, while a bus costs an average of €43.
Distance: 83 miles (135 km)
Most popular Bus | Driving |
|---|---|
€43 Average price Cheapest | €17 Average price (fuel + tolls) |
3h 51m Average total duration Fastest 3h 21m Onboard + 30m Additional time* | 2h 4m Average total duration (door-to-door) |
![]() Most popular bus company | Your own car or car rental |
Direct Options | Direct Options |
Tickets start at around €43 compared to roughly €17 in fuel and tolls — a saving of about €-26.42. The journey takes 3h 21m versus 2h 4m by car, so you'll arrive a bit later, but you can use the time productively rather than spending it behind the wheel. No parking to sort, no fuel stops, and most coaches have Wi-Fi and power outlets.
Driving is worth considering if you're on a tight schedule, travelling as a group (where fuel costs split several ways), or want the flexibility to stop along the way.
| Operator | Departure Time | Arrival Time | Duration | Departure days | Cheapest price |
|---|---|---|---|---|---|
Operator FlixBus | Departure Time 12:15AM | Arrival Time 05:05AM | Duration 4h50 | Departure days Wed, Sun | Cheapest price €40 |
Operator FlixBus | Departure Time 01:15AM | Arrival Time 06:05AM | Duration 4h50 | Departure days Wed | Cheapest price €37 |
Operator Greyhound | Departure Time 03:55AM | Arrival Time 11:50AM | Duration 7h55 | Departure days Thu | Cheapest price €41 |
Operator Greyhound | Departure Time 04:55AM | Arrival Time 12:50PM | Duration 7h55 | Departure days Thu | Cheapest price €36 |
Operator Flixbus USA | Departure Time 09:55AM | Arrival Time 03:35PM | Duration 5h40 | Departure days Mon, Thu, Fri, Sun | Cheapest price €25 |
Operator FlixBus | Departure Time 02:40PM | Arrival Time 07:35PM | Duration 4h55 | Departure days Tue | Cheapest price €42 |
Operator FlixBus | Departure Time 04:10PM | Arrival Time 09:55PM | Duration 5h45 | Departure days Tue | Cheapest price €56 |
Operator Flixbus USA | Departure Time 06:55PM | Arrival Time 12:00PM | Duration 17h05 | Departure days Thu, Fri, Sun | Cheapest price €25 |
The table below shows live departures for Daytona Beach, FL to Gainesville, FL buses for today, Friday, September 18. You can also manually refresh the results below to see updated bus journeys.
| Provider | Departs | Duration | Arrives | Transfers | Tickets |
|---|---|---|---|---|---|
![]() | 6:55 PM Daytona Beach, FL | 17h05 | 12:00 PM Gainesville, FL | 1 transfer | |
| Saturday, September 19 | |||||
![]() | 12:15 AM Daytona Beach, FL | 4h50 | 5:05 AM Gainesville, FL | 0 transfersDirect | |
This is the last bus of the day. | |||||
Find all the dates and times for this journey
Compare bus prices over the next three weeks. See the best deals at a glance, find the lowest fares, and choose the option that fits your budget.
Buses from Daytona Beach, FL to Gainesville, FL run on average 3 times per day, taking around 3h 21m. Cheap bus tickets for this journey start at €35 if you book in advance.
The earliest bus runs at 00:25, the last at 22:40. The fastest bus covers the 83 miles (135 km) distance in 2h 59m.
Distance 83 miles (135 km) |
Average bus duration 3h 21m |
Cheapest ticket price €35 |
Buses per day 3 |
Fastest bus 2h 59m |
First bus 12:25 AM |
Last bus 10:40 PM |
Cheapest Month Jun |
Cheapest Day Thu |
High Season Jan - Mar |
Low Season Oct - Dec |
Discover the best of Gainesville, FL — top attractions, local food, transport tips, budget advice, and currency essentials. Plan your perfect Gainesville, FL trip today.
Natural history museum with Florida fossils, butterfly exhibits, and family-friendly science displays on the University of Florida campus.
University art museum featuring global collections, rotating exhibitions, and strong photography and modern art presentations.
Tree-lined downtown district with historic buildings, murals, music venues, and an easy walk to shops, theaters, and public art.
Tree-lined downtown district with historic buildings, murals, music venues, and an easy walk to shops, theaters, and public art.
Large wetlands park with boardwalks, wildlife viewing, and broad views over marshes that attract birds, alligators, and photographers.
Lively student-centered district near UF with shops, nightlife, murals, and walkable streets that capture the city's college-town energy.
Find answers to the most common questions about traveling from Daytona Beach, FL to Gainesville, FL by bus. From journey times and ticket prices to direct connections, first and last departures, and even whether the route is scenic, our FAQs cover everything you need to plan your trip. Whether you’re looking for the fastest bus, the cheapest option, or tips for making the most of your visit at Gainesville, FL, this guide helps you travel smarter and with confidence.

Most Daytona Beach, FL to Gainesville, FL buses use the Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot) to Gainesville, Rosa Parks Transfer station station pair, with some services arriving at Gainesville, NW 23rd St (North Walmart Supercenter). Use this guide to compare Daytona Beach, FL and Gainesville, FL bus stations on this route, check city-centre transport, alongside checking practicalities like bus station facilities and services.
Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot) is 7 km from Daytona Beach city centre. Public transport options include:
Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot) has practical services for this route, including Wifi, Parking, Dining, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Food options include: Restaurants available inside the Tanger Outlets.
Wi-Fi is available at Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot). ATMs are aTMs available at the Tanger Outlets.
Ticket office support is available at Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot).
Available at Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot): Parking, Taxis, WC.
Accessibility facilities are available at Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot): Wheelchair accessible pathways available.
Daytona Beach, S Ridgewood Ave (Greyhound Bus Station) has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Free Wi-Fi available inside the station. ATMs are available at Daytona Beach, S Ridgewood Ave (Greyhound Bus Station).
Ticket office support is available at Daytona Beach, S Ridgewood Ave (Greyhound Bus Station).
Available at Daytona Beach, S Ridgewood Ave (Greyhound Bus Station): Parking, Taxis, WC.
Accessibility facilities are available at Daytona Beach, S Ridgewood Ave (Greyhound Bus Station): Wheelchair accessible entrance and facilities.
Gainesville, Rosa Parks Transfer station has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Free Wi-Fi is available at the station. ATMs are available at Gainesville, Rosa Parks Transfer station.
Ticket office support is available at Gainesville, Rosa Parks Transfer station.
Available at Gainesville, Rosa Parks Transfer station: Parking, Taxis, WC.
Accessibility facilities are available at Gainesville, Rosa Parks Transfer station: The station is wheelchair accessible.
Gainesville, NW 23rd St (North Walmart Supercenter) is 7 km from Gainesville city centre. Public transport options include:
Gainesville, NW 23rd St (North Walmart Supercenter) has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Wi-Fi is available at Gainesville, NW 23rd St (North Walmart Supercenter). ATMs are available at Gainesville, NW 23rd St (North Walmart Supercenter).
Ticket office support is available at Gainesville, NW 23rd St (North Walmart Supercenter).
Available at Gainesville, NW 23rd St (North Walmart Supercenter): Parking, Taxis, WC.
Accessibility facilities are available at Gainesville, NW 23rd St (North Walmart Supercenter): Bus stop has basic accessibility features.
Here are some other resources that might have the information you need