- Must visit
Florida Museum of Natural History
The state’s official natural history museum, known for Florida fossils, biodiversity exhibits, and family-friendly displays. A top cultural stop in Gainesville.
Cheapest price
$31
Average price
$31
Fastest journey
5 h 40 m
Average duration
11 h 23 m
Buses per day
2
Distance
135 km
The best way to find a cheap bus ticket from Daytona Beach, FL to Gainesville, FL is to book your journey as far in advance as possible and to avoid traveling at rush hour.
The average ticket from Daytona Beach, FL to Gainesville, FL will cost around $31 if you buy it on the day, but the cheapest tickets can be found for only $31.
It's difficult to get from Daytona Beach, FL to Gainesville, FL without transferring at least once.




Omio recommends booking the bus as it is the most popular option from Daytona Beach, FL to Gainesville, FL among Omio's users. If you are prioritising price take the bus with prices starting from $44. For those with little time consider booking a bus which can get you there in 3h 15m. The ticket price range depends on the travel mode: a bus costs an average of $44, and a bus costs an average of $44.
Distance: 83 miles (135 km)
Most popular Bus | Driving |
|---|---|
$52 Average price Cheapest | $20 Average price (fuel + tolls) |
3h 45m Average total duration Fastest 3h 15m Onboard + 30m Additional time* | 2h 4m Average total duration (door-to-door) |
![]() Most popular bus company | Your own car or car rental |
Direct Options | Direct Options |
You'll arrive about {timeSavings} sooner ({durationInMinutes} by bus vs {drivingDurationInMinutes} by car) and save roughly {savingsAmount} compared to fuel and tolls ({cheapestPrice} vs {drivingPriceInCents}). You also skip the drive itself — no traffic stress, no parking, and you can use the time to work, nap, or catch up on a series with the onboard Wi-Fi.
Direct coaches are operated by {directProviders}.
Driving is still worth considering if you're travelling with bulky luggage, need flexibility on departure times, or plan to stop along the way.
| Operator | Departure Time | Arrival Time | Duration | Departure days | Cheapest price |
|---|---|---|---|---|---|
Operator FlixBus | Departure Time 12:15AM | Arrival Time 05:05AM | Duration 4h50 | Departure days Wed, Sun | Cheapest price $48 |
Operator FlixBus | Departure Time 01:15AM | Arrival Time 06:05AM | Duration 4h50 | Departure days Wed | Cheapest price $45 |
Operator Greyhound | Departure Time 03:55AM | Arrival Time 11:50AM | Duration 7h55 | Departure days Thu | Cheapest price $51 |
Operator Greyhound | Departure Time 04:55AM | Arrival Time 12:50PM | Duration 7h55 | Departure days Thu | Cheapest price $44 |
Operator Flixbus USA | Departure Time 09:55AM | Arrival Time 03:35PM | Duration 5h40 | Departure days Mon, Thu, Fri, Sun | Cheapest price $31 |
Operator FlixBus | Departure Time 02:40PM | Arrival Time 07:35PM | Duration 4h55 | Departure days Tue | Cheapest price $51 |
Operator FlixBus | Departure Time 04:10PM | Arrival Time 09:55PM | Duration 5h45 | Departure days Tue | Cheapest price $69 |
Operator Flixbus USA | Departure Time 06:55PM | Arrival Time 12:00PM | Duration 17h05 | Departure days Thu, Fri, Sun | Cheapest price $31 |
Compare bus prices over the next three weeks. See the best deals at a glance, find the lowest fares, and choose the option that fits your budget.
Buses from Daytona Beach, FL to Gainesville, FL run on average 5 times per day, taking around 3h 15m. Cheap bus tickets for this journey start at $44 (€36) if you book in advance.
The earliest bus runs at 00:25, the last at 22:40. The fastest bus covers the 83 miles (135 km) distance in 4h 50m.
Distance 83 miles (135 km) |
Average bus duration 3h 15m |
Cheapest ticket price $44 (€36) |
Buses per day 5 |
Fastest bus 4h 50m |
First bus 12:25 AM |
Last bus 10:40 PM |
Cheapest Month Feb |
Cheapest Day Thu |
High Season Jan - Mar |
Low Season Apr - Jun |
Discover the best of Gainesville — top attractions, local food, transport tips, budget advice, and currency essentials. Plan your perfect Gainesville trip today.
The state’s official natural history museum, known for Florida fossils, biodiversity exhibits, and family-friendly displays. A top cultural stop in Gainesville.
A major university art museum with strong Asian, African, modern, and contemporary collections. Highly regarded for its quality exhibitions and free admission.
Interactive science and invention museum inspired by innovation, featuring hands-on exhibits for adults and children. Popular with families and curious visitors.
One of Gainesville’s most popular public spaces, with walking paths, lawns, water features, and frequent community activity. Great for relaxing and photos.
A walkable area with red-brick buildings, mature trees, Century Tower, and classic collegiate atmosphere. Ideal for a first visit to Gainesville.
A major natural area near Gainesville known for sweeping views, wildlife, trails, and observation opportunities. One of the region’s signature outdoor experiences.
A North Florida specialty made from alligator tail meat, usually fried or grilled. It is a regional novelty tied to Florida’s wetlands and game cuisine.
Slow-smoked pork or beef served with classic sides such as beans, slaw, and cornbread. It is a staple comfort food across North Florida.
Slices of unripe tomato coated and fried until crisp. This Southern favorite is common in North Florida restaurants and farm-style cooking.
A well-known upscale steakhouse specializing in wood-grilled meats, seafood, and polished service in an elegant setting.
A beloved Gainesville institution serving pizza, salads, and beer in a quirky, family-friendly space filled with local character.
An established Japanese restaurant in downtown Gainesville known for sushi, robata dishes, and a stylish upscale setting.
Moderate by U.S. standards. Student demand keeps basics reasonable, while hotels and downtown dining can cost more on busy weekends.
Tip 18-20% at sit-down restaurants; 20%+ for great service. USD 1-2 per drink at bars, round up taxis, and tip delivery USD 3-5 or 15-20%.
Find answers to the most common questions about traveling from Daytona Beach, FL to Gainesville, FL by bus. From journey times and ticket prices to direct connections, first and last departures, and even whether the route is scenic, our FAQs cover everything you need to plan your trip. Whether you’re looking for the fastest bus, the cheapest option, or tips for making the most of your visit at Gainesville, FL, this guide helps you travel smarter and with confidence.

Most Daytona Beach, FL to Gainesville, FL buses use the Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot) to Gainesville, Rosa Parks Transfer station station pair, with some services arriving at Gainesville, NW 23rd St (North Walmart Supercenter). Use this guide to compare Daytona Beach, FL and Gainesville, FL bus stations on this route, check city-centre transport, alongside checking practicalities like bus station facilities and services.
Here are some other resources that might have the information you need