- Must visit
Florida Museum of Natural History
Natural history museum with Florida fossils, butterfly exhibits, and family-friendly science displays on the University of Florida campus.
The best way to find a cheap bus ticket from Daytona Beach, FL to Gainesville, FL is to book your journey as far in advance as possible and to avoid traveling at rush hour.
The average ticket from Daytona Beach, FL to Gainesville, FL will cost around €46 if you buy it on the day, but the cheapest tickets can be found for only €37.
On Wed, Nov 04, we found 4 buses from Daytona Beach, FL to Gainesville, FL, including 4 direct services.
On Wed, Nov 04 the direct buses cover the 135 km distance in an average of 5 h 5 m but if you time it right, some buses will get you there in just 4 h 50 m .
On Wed, Nov 04 the slowest buses will take 5 h 45 m and usually involve a change or two along the way, but you might be able to save a few pennies if you’re on a budget.

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Buses from Daytona Beach, FL to Gainesville, FL run on average 3 times per day, taking around 3h 27m. Cheap bus tickets for this journey start at €29 if you book in advance.
The earliest bus runs at 00:25, the last at 22:40. The fastest bus covers the 83 miles (135 km) distance in 2h 59m.
Plan this route
Use these quick route answers to check fares, travel time, departure options, and direct services before booking from Daytona Beach, FL to Gainesville, FL.
| Operator | Departure Time | Arrival Time | Duration | Departure days | Cheapest price |
|---|---|---|---|---|---|
Operator FlixBus | Departure Time 12:15AM | Arrival Time 05:05AM | Duration 4h50 | Departure days Wed, Sun | Cheapest price €40 |
Operator Greyhound | Departure Time 12:50AM | Arrival Time 07:50AM | Duration 7h00 | Departure days Fri | Cheapest price €53 |
Operator FlixBus | Departure Time 01:15AM | Arrival Time 06:05AM | Duration 4h50 | Departure days Wed | Cheapest price €37 |
Operator Greyhound | Departure Time 03:55AM | Arrival Time 11:50AM | Duration 7h55 | Departure days Thu | Cheapest price €42 |
Operator Greyhound | Departure Time 04:55AM | Arrival Time 12:50PM | Duration 7h55 | Departure days Thu | Cheapest price €36 |
Operator FlixBus | Departure Time 07:20AM | Arrival Time 11:59AM | Duration 4h39 | Departure days Fri | Cheapest price €53 |
Operator Flixbus USA | Departure Time 09:55AM | Arrival Time 03:35PM | Duration 5h40 | Departure days Mon, Thu, Sun | Cheapest price €25 |
Operator FlixBus | Departure Time 10:40AM | Arrival Time 03:35PM | Duration 4h55 | Departure days Fri | Cheapest price €53 |
Operator FlixBus | Departure Time 11:05AM | Arrival Time 05:00PM | Duration 5h55 | Departure days Fri | Cheapest price €54 |
Operator FlixBus | Departure Time 01:10PM | Arrival Time 05:00PM | Duration 3h50 | Departure days Fri | Cheapest price €53 |
Operator FlixBus | Departure Time 02:40PM | Arrival Time 07:35PM | Duration 4h55 | Departure days Tue | Cheapest price €42 |
Operator FlixBus | Departure Time 04:10PM | Arrival Time 09:55PM | Duration 5h45 | Departure days Tue | Cheapest price €57 |
Operator Flixbus USA | Departure Time 06:55PM | Arrival Time 12:00PM | Duration 17h05 | Departure days Thu, Sun | Cheapest price €25 |
Operator FlixBus | Departure Time 08:15PM | Arrival Time 11:55PM | Duration 3h40 | Departure days Fri | Cheapest price €53 |
Operator FlixBus | Departure Time 10:55PM | Arrival Time 03:05AM | Duration 4h10 | Departure days Fri | Cheapest price €53 |
The table below shows live departures for Daytona Beach, FL to Gainesville, FL buses for tomorrow, Tuesday, November 3. You can also manually refresh the results below to see updated bus journeys.
| Provider | Departs | Duration | Arrives | Transfers | Tickets |
|---|---|---|---|---|---|
![]() | 12:15 AM Daytona Beach, FL | 4h50 | 5:05 AM Gainesville, FL | 0 transfersDirect | |
![]() | 1:15 AM Daytona Beach, FL | 4h50 | 6:05 AM Gainesville, FL | 0 transfersDirect | |
![]() | 2:40 PM Daytona Beach, FL | 4h55 | 7:35 PM Gainesville, FL | 0 transfersDirect | |
![]() | 4:10 PM Daytona Beach, FL | 5h45 | 9:55 PM Gainesville, FL | 0 transfersDirect | |
This is the last bus of the day. | |||||
Find all the dates and times for this journey
Buses from Daytona Beach, FL to Gainesville, FL with FlixBus and Greyhound. Overnight buses available on this route with FlixBus and Greyhound. Use the details below to compare onboard facilities.
Review the details behind your booking, from operators and ticket rules to luggage, onboard amenities, accessibility, and route-specific travel policies.
Discover the best of Gainesville, FL — top attractions, local food, transport tips, budget advice, and currency essentials. Plan your perfect Gainesville, FL trip today.
Natural history museum with Florida fossils, butterfly exhibits, and family-friendly science displays on the University of Florida campus.
University art museum featuring global collections, rotating exhibitions, and strong photography and modern art presentations.
Tree-lined downtown district with historic buildings, murals, music venues, and an easy walk to shops, theaters, and public art.
Tree-lined downtown district with historic buildings, murals, music venues, and an easy walk to shops, theaters, and public art.
Large wetlands park with boardwalks, wildlife viewing, and broad views over marshes that attract birds, alligators, and photographers.
Lively student-centered district near UF with shops, nightlife, murals, and walkable streets that capture the city's college-town energy.
North Florida barbecue often features slow-smoked pork or brisket with tangy sauces, reflecting Southern pit traditions common at local gatherings and roadside smokehouses.
Fresh Gulf shrimp are a Florida staple, commonly boiled, fried, or served in baskets. Gainesville restaurants highlight this regional seafood from nearby Gulf waters.
This Florida classic layers tart Key lime juice, sweetened condensed milk, and a crumb crust. It is a signature state dessert found widely in Gainesville.
Long-running barbecue spot serving smoked meats, classic sides, and a casual local atmosphere near downtown.
Stylish downtown venue focused on modern Southern food, oysters, and cocktails in an energetic setting.
Refined Southern restaurant known for seasonal plates, cocktails, and a polished but relaxed downtown setting.
Moderate for U.S. college towns: hotels can rise on game days, dining is reasonable, and most visitors need a car or rideshares.
Tip 18-20% at restaurants, 15-20% for taxis and rideshares, and $1-2 per drink or coffee is appreciated.
Get destination-focused answers for the end of your trip, including what to do, when to go, practical entry details, and tips for making the most of your time in Gainesville, FL.
Find answers to the most common questions about traveling from Daytona Beach, FL to Gainesville, FL by bus. From journey times and ticket prices to direct connections, first and last departures, and even whether the route is scenic, our FAQs cover everything you need to plan your trip. Whether you’re looking for the fastest bus, the cheapest option, or tips for making the most of your visit at Gainesville, FL, this guide helps you travel smarter and with confidence.

Most Daytona Beach, FL to Gainesville, FL buses use the Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot) to Gainesville, Rosa Parks Transfer station station pair, with some services arriving at Gainesville, NW 23rd St (North Walmart Supercenter). Use this guide to compare Daytona Beach, FL and Gainesville, FL bus stations on this route, check city-centre transport, alongside checking practicalities like bus station facilities and services.
Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot) is 7 km from Daytona Beach city centre. Public transport options include:
Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot) has practical services for this route, including Wifi, Parking, Dining, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Food options include: Restaurants available inside the Tanger Outlets.
Wi-Fi is available at Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot). ATMs are aTMs available at the Tanger Outlets.
Ticket office support is available at Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot).
Available at Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot): Parking, Taxis, WC.
Accessibility facilities are available at Daytona Beach, Cornerstone Blvd ("Tanger Outlets" parking lot): Wheelchair accessible pathways available.
Daytona Beach, S Ridgewood Ave (Greyhound Bus Station) has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Free Wi-Fi available inside the station. ATMs are available at Daytona Beach, S Ridgewood Ave (Greyhound Bus Station).
Ticket office support is available at Daytona Beach, S Ridgewood Ave (Greyhound Bus Station).
Available at Daytona Beach, S Ridgewood Ave (Greyhound Bus Station): Parking, Taxis, WC.
Accessibility facilities are available at Daytona Beach, S Ridgewood Ave (Greyhound Bus Station): Wheelchair accessible entrance and facilities.
Gainesville, Rosa Parks Transfer station has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Free Wi-Fi is available at the station. ATMs are available at Gainesville, Rosa Parks Transfer station.
Ticket office support is available at Gainesville, Rosa Parks Transfer station.
Available at Gainesville, Rosa Parks Transfer station: Parking, Taxis, WC.
Accessibility facilities are available at Gainesville, Rosa Parks Transfer station: The station is wheelchair accessible.
Gainesville, NW 23rd St (North Walmart Supercenter) is 7 km from Gainesville city centre. Public transport options include:
Gainesville, NW 23rd St (North Walmart Supercenter) has practical services for this route, including Wifi, Parking, ATM, Taxis, Accessibility, WC, Shower, and Ticket Office.
Wi-Fi is available at Gainesville, NW 23rd St (North Walmart Supercenter). ATMs are available at Gainesville, NW 23rd St (North Walmart Supercenter).
Ticket office support is available at Gainesville, NW 23rd St (North Walmart Supercenter).
Available at Gainesville, NW 23rd St (North Walmart Supercenter): Parking, Taxis, WC.
Accessibility facilities are available at Gainesville, NW 23rd St (North Walmart Supercenter): Bus stop has basic accessibility features.
Check bus stations information for Daytona Beach, FL and Gainesville, FL, including where buses depart and arrive on this route.